Larger substrates will require more solution. Only need to use 1-2 ml of your 10 ml syringe. Remove the cap from the needle, and insert it through the injection point of the substrate. Wipe the injection point on your substrate with alcohol. Make sure you shake the liquid culture syringe to disperse the mycelium. Put on your gloves and wipe them with rubbing alcohol.Ĭarefully open the packaging that your liquid culture syringe came in, remove the cap on the end of Leave your work area for a few minutes to let the disinfectant settle. Surface, your substrate and syringe with rubbing alcohol and spray an air sanitizing spray like Oust or Make sure your hands are clean, and you are wearing clean, freshly laundered clothesĬlean your work area thoroughly with a disinfectant such as bleach or Lysol. Needle, alcohol wipes, rubber gloves, face mask and instructions.
Package should contain: live liquid culture syringe, sterile packaged Work should be done in front of a laminar flow hood or glove box in theĬleanest environment possible.
SPORE DARK INJECTION SPORE API HOW TO
How to inoculate and cultivate your substrate: Notice: Do not use the mushroom spore syringe in combination with Liquid Culture! If you want to make a Liquid Culture from your mushroom spores choose the spore vial or spore print instead of the spore syringe. Reattach the luer lock to the syringe after use. Store the spore syringe refrigerated between 2*C-8*C Celsius or 35*F-46*F Fahrenheit. Make a spore print form the tallest and fattest mushroom and you will have a good specimen for further research. When the mushrooms (fruits of the mycelium) start to grow and mature, they can be picked and their spores can be harvest for preservation. Wait until the whole substrate is colonized by the mushrooms mycelium before you start the fruiting process. Just drop a few milliliter in every corner of the mushroom substrate and you are ready to go.Īfter a few days white patches of mycelium will start growing on the places were the spores were dropped. A syringe contains 20 ml of spore solution, enough to inoculate 6-7 liters of substrate. With the mushroom spores syringe you will only need to drop 3 milliliter of spore solution to colonize 1 liter or 1.05 quart of substrate. Edible, medicinal or psilocybe mushroom strains need different substrates to grow on. This can be done by inoculating a substrate, a soil with nutritions specially developed for growing mushrooms. If you want to reproduce spores it will be needed to fruit some mushrooms from the chosen mushroom spores. The inoculation of substrate with spores syringe Have a look at the shape,size and color of the spores and what kind you have. Take a cover glass and put this on the drop of spores. Shake the spore syringe and add one drop of spore solution on to the microscope slide. Get a microscope slide and clean it properly. The spore syringe is now ready for further use, like microscopic research or ready to inoculate a sterile substrate. Before use shake the spore syringe for a few seconds. Remove the needle from it's sterile package (leave the protection cap on the needle) and attach it to the spore syringe. Unscrew the the cap from the spore syringe by turning it counter clockwise. Hold the syringe with the cap pointing up. Take spore syringe with the cap still on it. The syringe is sealed with a cap, called luer lock. 1x special spore solution in a 20ml syringe.Our mushroom spore syringe comes in a pack that includes: When the spore syringe was stored in the refrigerator it is best to take it out 12 hours before use. The pre-made spore syringes are ready to use. Spore syringe instructions: How to use a mushroom spore syringe ?įirst make sure that your working area is clean, disinfect your hands or use sterile gloves before you prepare your mushroom spore syringe. Werther you choose to grow Oyster mushrooms, magic mushrooms or a medicinal strain a spore syringe is used to start the cultivating process. The mushroom spore syringe is used to inoculate substrate, a nutritious grow environment, with a mushroom strain of choice. A mushroom spore syringe is easy use and store. One syringe contains millions of quality mushroom spores, floating in a sterile solution.
Spore syringes are a commonly used medium that contain spores from any kind of mushroom strain.